Ion 530™ ჩიპის ნაკრები

კატალოგის ნომერი: A34019 Ion 510 & Ion 520 & Ion 530 Chef-ნაკრები (1 სექვენირების რეაქცია თითოეული ინიციალიზაციისას) საშუალებას იძლევა ნიმუშის მომზადება განხორციელდეს ავტომატურად. Ion Chef სისტემით მომზადებული რიდების სიგრძე აღნიშნული ნაკრების შემთხვევაში შეადგენს 400 აზოტოვან ფუძეს, რომელთა სექვენირება ხორციელდება Ion S5 ან Ion S5 XL სექვენირების პლატფორმების საშუალებით. Ion 510/Ion 520/Ion 530 ნაკრები მოიცავს ერთჯერადი გამოყენებისთვის წიანასწარ მომზადებულ სექვენირების რეაქგენტების კარტრიჯს, რომელსაც ინტეგრირებული აქვს ნიმუშის კონტროლის, მიწოდების და ავტომატური პროტოკოლის მართვის ფუნქციები. ნაკრებით შესაძლებელია ჩიპებზე დატანილი 8 ნიმუშის სექვენირება. რეაქციის ყოველი ინიციალიზაციისას შესაძლებელია 1 სექვენირების რეაქციის ჩატარება. სექვენირების 2 რეაქციის გასაშვებად თითოეული ინიციალიზაციისას (200 აზოტოვანი ფუძე) იხილეთ ნაკრები: No. A34461. Ion 510 / Ion 520 / Ion 530 Chef - ნაკრები:

• ნიმუშის მომზადება და ჩიპში ჩატვირთვა 400 აზოტოვან ფუძემდე სიგძის მქონე რიდებისთვის;
• სექვენირება გამარტივებულია ერთჯერადი, გამოყენებისთვის მზა ფორმის რეაგენტების კარტრიჯებით;
• სწრაფი და მარტივი სექვენირება, 15 წუთზე ნაკლები დრო ხელით სამუშაოდ;
• მაქსიმალური მოქნილობა ჩიპებთან თავსებადობის მხრივ: Ion 510, Ion 520, და Ion 530 ჩიპები.

Catalog number:  A27828 Scalable isolation of RNA (including small RNAs) The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays. The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand. Features of the MagMAX mirVana Total RNA Isolation Kit include:

• Automation-ready, phenol-free extraction
• Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
• Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples. The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

• Serum/plasma
• Whole blood
• Tissue
• Cell culture
• Urine

Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Catalog number:  AM1835 Applied Biosystems® This kit is for the isolation of viral RNA and DNA from diverse sample types (i.e., environmental samples and biofluids). Nucleic acids purified using this kit are ideal for PCR and RT-PCR applications. This is a first generation MagMAX™ Viral RNA Isolation Kit validated by the NVSL for use with AI/ND viruses. Perfect for working with low viral concentrations, the kit contains sufficient reagents for 4 x 96 reactions.

• Validated by the NVSL for use with AI/ND viruses
• Recover as few as 10 copies of viral RNA from 100–400 µL sample
• Typical viral RNA recovery of >50%
• Ideal when working with low viral concentrations
• Linear recovery down to 50 transcripts
• 96 samples can be processed in 30 minutes
• Easily integrated into robotic platforms

Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples. Optimized for Isolation of Virus from Biofluids: The Applied Biosystems® MagMAX™ Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA isolation kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields.

Catalog number:  Q32855 The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Which product to choose for RNA HS (High Sensitivity) quantitation?

• For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate reader